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Dual gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae
BACKGROUND: Manipulations in Saccharomyces cerevisiae classically depend on use of auxotrophy selection markers. There are several disadvantages to this in a microbial cell factory setting: (1) auxotrophies must first be engineered in prototrophic strains, and many industrial strains are polyploid/a...
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Publicado no: | Microb Cell Fact |
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Main Authors: | , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
BioMed Central
2013
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4231455/ https://ncbi.nlm.nih.gov/pubmed/24161108 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-12-96 |
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