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Dual gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae

BACKGROUND: Manipulations in Saccharomyces cerevisiae classically depend on use of auxotrophy selection markers. There are several disadvantages to this in a microbial cell factory setting: (1) auxotrophies must first be engineered in prototrophic strains, and many industrial strains are polyploid/a...

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Bibliografiske detaljer
Udgivet i:Microb Cell Fact
Main Authors: Vickers, Claudia E, Bydder, Sarah F, Zhou, Yuchan, Nielsen, Lars K
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4231455/
https://ncbi.nlm.nih.gov/pubmed/24161108
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-12-96
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