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Determination of Glutamate Dehydrogenase Activity and Its Kinetics in Mouse Tissues using Metabolic Mapping (Quantitative Enzyme Histochemistry)

Glutamate dehydrogenase (GDH) catalyses the reversible conversion of glutamate into α-ketoglutarate with the concomitant reduction of NAD(P)(+) to NAD(P)H or vice versa. GDH activity is subject to complex allosteric regulation including substrate inhibition. To determine GDH kinetics in situ, we ass...

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Veröffentlicht in:J Histochem Cytochem
Hauptverfasser: Botman, Dennis, Tigchelaar, Wikky, Van Noorden, Cornelis J.F.
Format: Artigo
Sprache:Inglês
Veröffentlicht: SAGE Publications 2014
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4230541/
https://ncbi.nlm.nih.gov/pubmed/25124006
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1369/0022155414549071
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