Purification and Properties of the Cathepsin D Type Proteinase from Beef and Rabbit Lung and Its Identification in Macrophages

The acid-acting proteinase, cathepsin D (EC 3.4.4.23), was purified from extracts of homogenized rabbit lung and beef lung by autolysis at acid pH, acetone and ammonium sulfate fractionation, column chromatography, and isoelectric focusing. Four isoenzymes were obtained from each source. With acid h...

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Main Authors: Rojas-Espinosa, Oscar, Dannenberg, Arthur M., Murphy, Patrick A., Straat, Patricia A., Huang, P. C., James, Stephen P.
Format: Artigo
Sprog:Inglês
Udgivet: 1973
Fag:
Pathogenic Mechanisms, Ecology, and Epidemiology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC422962/
https://ncbi.nlm.nih.gov/pubmed/4784884
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