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Laurdan monitors different lipids content in eukaryotic membrane during embryonic neural development

We describe a method based on fluorescence lifetime imaging microscopy (FLIM) to assess the fluidity of various membranes in neuronal cells at different stage of development (day 12 (E12) and day 16 (E16) of gestation). For the FLIM measurements, we use the Laurdan probe which is commonly used to as...

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Detalhes bibliográficos
Publicado no:Cell Biochem Biophys
Main Authors: Bonaventura, Gabriele, Barcellona, Maria Luisa, Golfetto, Ottavia, Nourse, Jamison L., Flanagan, Lisa A., Gratton, Enrico
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4228983/
https://ncbi.nlm.nih.gov/pubmed/24839062
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12013-014-9982-8
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