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Detrimental effect of the 6 His C-terminal tag on YedY enzymatic activity and influence of the TAT signal sequence on YedY synthesis

BACKGROUND: YedY, a molybdoenzyme belonging to the sulfite oxidase family, is found in most Gram-negative bacteria. It contains a twin-arginine signal sequence that is cleaved after its translocation into the periplasm. Despite a weak reductase activity with substrates such as dimethyl sulfoxide or...

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Publicado en:BMC Biochem
Main Authors: Sabaty, Monique, Grosse, Sandrine, Adryanczyk, Geraldine, Boiry, Séverine, Biaso, Frédéric, Arnoux, Pascal, Pignol, David
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2013
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4228395/
https://ncbi.nlm.nih.gov/pubmed/24180491
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2091-14-28
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