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High-Efficiency Genome Editing and Allele Replacement in Prototrophic and Wild Strains of Saccharomyces
Current genome editing techniques available for Saccharomyces yeast species rely on auxotrophic markers, limiting their use in wild and industrial strains and species. Taking advantage of the ancient loss of thymidine kinase in the fungal kingdom, we have developed the herpes simplex virus thymidine...
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| Publicado no: | Genetics |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Genetics Society of America
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4224175/ https://ncbi.nlm.nih.gov/pubmed/25209147 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.114.170118 |
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