Development of a Blocking ELISA Based on a Monoclonal Antibody against a Predominant Epitope in Non-Structural Protein 3B2 of Foot-and-Mouth Disease Virus for Differentiating Infected from Vaccinated Animals

A monoclonal antibody (McAb) against non-structural protein (NSP) 3B of foot-mouth-disease virus (FMDV) (3B4B1) was generated and shown to recognize a conserved epitope spanning amino acids 24–32 of 3B (GPYAGPMER) by peptide screening ELISA. This epitope was further shown to be a unique and predomin...

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Veröffentlicht in:PLoS One
Hauptverfasser: Fu, Yuanfang, Lu, Zengjun, Li, Pinghua, Cao, Yimei, Sun, Pu, Tian, Meina, Wang, Na, Bao, Huifang, Bai, Xingwen, Li, Dong, Chen, Yingli, Liu, Zaixin
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2014
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4219772/
https://ncbi.nlm.nih.gov/pubmed/25369323
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0111737
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