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Point Mutations in Transmembrane Helices 2 and 3 of ExbB and TolQ Affect Their Activities in Escherichia coli K-12

Replacement of glutamate 176, the only charged amino acid in the third transmembrane helix of ExbB, with alanine (E176A) abolished ExbB activity in all determined ExbB-dependent functions of Escherichia coli. Combination of the mutations T148A in the second transmembrane helix and T181A in the third...

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Detalhes bibliográficos
Main Authors: Braun, Volkmar, Herrmann, Christina
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC421596/
https://ncbi.nlm.nih.gov/pubmed/15205446
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.186.13.4402-4406.2004
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