A Toxoplasma gondii Class XIV Myosin, Expressed in Sf9 Cells with a Parasite Co-chaperone, Requires Two Light Chains for Fast Motility

Many diverse myosin classes can be expressed using the baculovirus/Sf9 insect cell expression system, whereas others have been recalcitrant. We hypothesized that most myosins utilize Sf9 cell chaperones, but others require an organism-specific co-chaperone. TgMyoA, a class XIVa myosin from the paras...

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Autors principals: Bookwalter, Carol S., Kelsen, Anne, Leung, Jacqueline M., Ward, Gary E., Trybus, Kathleen M.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Biochemistry and Molecular Biology 2014
Matèries:
Protein Structure and Folding
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4215259/
https://ncbi.nlm.nih.gov/pubmed/25231988
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M114.572453
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