Quantitatively Imaging Chromosomes by Correlated Cryo-Fluorescence and Soft X-Ray Tomographies

Soft x-ray tomography (SXT) is increasingly being recognized as a valuable method for visualizing and quantifying the ultrastructure of cryopreserved cells. Here, we describe the combination of SXT with cryogenic confocal fluorescence tomography (CFT). This correlative approach allows the incorporat...

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Detalhes bibliográficos
Main Authors: Smith, Elizabeth A., McDermott, Gerry, Do, Myan, Leung, Karen, Panning, Barbara, Le Gros, Mark A., Larabell, Carolyn A.
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2014
Assuntos:
Systems Biophysics
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4213715/
https://ncbi.nlm.nih.gov/pubmed/25418180
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2014.09.011
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