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Production and Isolation of Axons from Sensory Neurons for Biochemical Analysis Using Porous Filters
Neuronal axons use specific mechanisms to mediate extension, maintain integrity, and induce degeneration. An appropriate balance of these events is required to shape functional neuronal circuits. The protocol described here explains how to use cell culture inserts bearing a porous membrane (filter)...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4212973/ https://ncbi.nlm.nih.gov/pubmed/25046441 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51795 |
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