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Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer
Assays based on Bioluminescence Resonance Energy Transfer (BRET) provide a sensitive and reliable means to monitor protein-protein interactions in live cells. BRET is the non-radiative transfer of energy from a 'donor' luciferase enzyme to an 'acceptor' fluorescent protein. In th...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4207229/ https://ncbi.nlm.nih.gov/pubmed/24893771 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51438 |
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