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Recombinatorial Cloning Using Heterologous Lox Sites

Recombination systems based on λ and Cre/loxP have been described to facilitate gene transfer from one vector to another in a high-throughput fashion, avoiding the bottlenecks associated with traditional cloning. However, no system described to date is suitable for the cloning of affinity reagents s...

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Autors principals: Siegel, Robert W., Velappan, Nileena, Pavlik, Peter, Chasteen, Leslie, Bradbury, Andrew
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 2004
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC419790/
https://ncbi.nlm.nih.gov/pubmed/15173117
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.1821804
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