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Verifying the Stability of Selected Genes for Normalization in Q PCR Experiments of Spodoptera frugiperda Cells during AcMNPV Infection

It is challenging to find genes with stable transcripts for use as reference genes for quantitative realtime polymerase chain reaction (qRT-PCR) during viral infection. Autographa californica nucleopolyhedrovirus (AcMNPV) is known to globally shut off host gene transcription in Sf21 cells and to mod...

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Bibliografske podrobnosti
Main Authors: Salem, Tamer Z., Allam, Walaa R., Thiem, Suzanne M.
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2014
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4196776/
https://ncbi.nlm.nih.gov/pubmed/25313905
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0108516
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