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Bacterially produced Pt-GFP as ratiometric dual-excitation sensor for in planta mapping of leaf apoplastic pH in intact Avena sativa and Vicia faba

BACKGROUND: Ratiometric analysis with H(+)-sensitive fluorescent sensors is a suitable approach for monitoring apoplastic pH dynamics. For the acidic range, the acidotropic dual-excitation dye Oregon Green 488 is an excellent pH sensor. Long lasting (hours) recordings of apoplastic pH in the near ne...

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Detalhes bibliográficos
Main Authors: Geilfus, Christoph-Martin, Mühling, Karl H, Kaiser, Hartmut, Plieth, Christoph
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4194361/
https://ncbi.nlm.nih.gov/pubmed/25313311
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1746-4811-10-31
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