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Finding the bottom and using it: Offsets and sensitivity in the detection of low intensity values in vivo with 2-photon microscopy

Maximizing 2-photon parameters used in acquiring images for quantitative intravital microscopy, especially when high sensitivity is required, remains an open area of investigation. Here we present data on correctly setting the black level of the photomultiplier tube amplifier by adjusting the offset...

詳細記述

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書誌詳細
主要な著者: Sandoval, Ruben M., Wang, Exing, Molitoris, Bruce A.
フォーマット: Artigo
言語:Inglês
出版事項: Taylor & Francis 2014
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC4194064/
https://ncbi.nlm.nih.gov/pubmed/25313346
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/intv.23674
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