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Identification of differentially expressed genes during development of the zebrafish pineal complex using RNA sequencing

We describe a method for isolating RNA suitable for high-throughput RNA sequencing (RNA-seq) from small numbers of fluorescently labeled cells isolated from live zebrafish (Danio rerio) embryos without using costly, commercially available columns. This method ensures high cell viability after dissoc...

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Main Authors: Khuansuwan, Sataree, Gamse, Joshua T.
格式: Artigo
語言:Inglês
出版: 2014
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4190028/
https://ncbi.nlm.nih.gov/pubmed/25173875
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ydbio.2014.08.015
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