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Quantifying distortions in two-photon remote focussing microscope images using a volumetric calibration specimen

Remote focussing microscopy allows sharp, in-focus images to be acquired at high speed from outside of the focal plane of an objective lens without any agitation of the specimen. However, without careful optical alignment, the advantages of remote focussing microscopy could be compromised by the int...

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Detalhes bibliográficos
Main Authors: Corbett, Alexander D., Burton, Rebecca A. B., Bub, Gil, Salter, Patrick S., Tuohy, Simon, Booth, Martin J., Wilson, Tony
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4189333/
https://ncbi.nlm.nih.gov/pubmed/25339910
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fphys.2014.00384
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