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Facilitating Protein Disulfide Mapping by a Combination of Pepsin Digestion, Electron Transfer Higher Energy Dissociation (EThcD), and a Dedicated Search Algorithm SlinkS

Disulfide bond identification is important for a detailed understanding of protein structures, which directly affect their biological functions. Here we describe an integrated workflow for the fast and accurate identification of authentic protein disulfide bridges. This novel workflow incorporates a...

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Detalhes bibliográficos
Main Authors: Liu, Fan, van Breukelen, Bas, Heck, Albert J. R.
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4189002/
https://ncbi.nlm.nih.gov/pubmed/24980484
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.O114.039057
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