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Cloning, expression, purification and preliminary X-ray analysis of EstN2, a novel archaeal α/β-hydrolase from Candidatus Nitrososphaera gargensis

EstN2 is a novel α/β-hydrolase originating from the ammonia-oxidizing thaumarchaeon Candidatus Nitrososphaera gargensis. The genome of the organism was sequenced and genes conferring putative lipolytic activity were amplified and cloned into Escherichia coli as a heterologous host. Through function-...

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מידע ביבליוגרפי
Main Authors: Kaljunen, Heidi, Chow, Jennifer, Streit, Wolfgang R., Mueller-Dieckmann, Jochen
פורמט: Artigo
שפה:Inglês
יצא לאור: International Union of Crystallography 2014
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC4188087/
https://ncbi.nlm.nih.gov/pubmed/25286947
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X14018482
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