Label-Free and Sensitive Fluorescent Detection of Sequence-Specific Single-Strand DNA Based on S1 Nuclease Cleavage Effects

The ability to detect sequence-specific single-strand DNA (ssDNA) in complex, contaminant-ridden samples, using a fluorescent method directly without a DNA extraction and PCR step could simplify the detection of pathogens in the field and in the clinic. Here, we have demonstrated a simple label-free...

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Bibliographic Details
Main Authors: Guan, Zheng, Liu, Jinchuan, Bai, Wenhui, Lv, Zhenzhen, Jiang, Xiaoling, Yang, Shuming, Chen, Ailiang, Lv, Guiyuan
Format: Artigo
Language:Inglês
Published: Public Library of Science 2014
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4186753/
https://ncbi.nlm.nih.gov/pubmed/25285445
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0108401
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