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Effect of β-lactam antibiotics on plant regeneration in carrot protoplast cultures

Protoplasts of three carrot cultivars were isolated from in vitro-grown plantlets by overnight incubation in an enzyme mixture composed of 1% (w/v) cellulase Onozuka R-10 and 0.1% (w/v) pectolyase Y-23. After cell immobilization in modified thin alginate layers, three types of β-lactam antibiotics (...

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Detalhes bibliográficos
Main Authors: Grzebelus, Ewa, Skop, Lukasz
Formato: Artigo
Idioma:Inglês
Publicado em: Springer US 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4182649/
https://ncbi.nlm.nih.gov/pubmed/25298730
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11627-014-9626-0
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