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Considerations for accurate gene expression measurement by reverse transcription quantitative PCR when analysing clinical samples

Reverse transcription quantitative PCR is an established, simple and effective method for RNA measurement. However, technical standardisation challenges combined with frequent insufficient experimental detail render replication of many published findings challenging. Consequently, without adequate c...

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Bibliografiske detaljer
Main Authors: Sanders, Rebecca, Mason, Deborah J., Foy, Carole A., Huggett, Jim F.
Format: Artigo
Sprog:Inglês
Udgivet: Springer Berlin Heidelberg 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4182594/
https://ncbi.nlm.nih.gov/pubmed/24858468
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00216-014-7857-x
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