Formation of brome mosaic virus RNA-dependent RNA polymerase in yeast requires coexpression of viral proteins and viral RNA.

In this report we show that yeast expressing brome mosaic virus (BMV) replication proteins 1a and 2a and replicating a BMV RNA3 derivative can be extracted to yield a template-dependent BMV RNA-dependent RNA polymerase (RdRp) able to synthesize (-)-strand RNA from BMV (+)-strand RNA templates added...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Quadt, R, Ishikawa, M, Janda, M, Ahlquist, P
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1995
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC41813/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7761419/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.92.11.4892
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