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Combining Rational and Random Strategies in β-Glucosidase Zm-p60.1 Protein Library Construction
Saturation mutagenesis is a cornerstone technique in protein engineering because of its utility (in conjunction with appropriate analytical techniques) for assessing effects of varying residues at selected positions on proteins’ structures and functions. Site-directed mutagenesis with degenerate pri...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4178128/ https://ncbi.nlm.nih.gov/pubmed/25260034 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0108292 |
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