Semi-Automated Hydrophobic Interaction Chromatography Column Scouting Used in the Two-Step Purification of Recombinant Green Fluorescent Protein

BACKGROUND: Hydrophobic interaction chromatography (HIC) most commonly requires experimental determination (i.e., scouting) in order to select an optimal chromatographic medium for purifying a given target protein. Neither a two-step purification of untagged green fluorescent protein (GFP) from crud...

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Bibliografiske detaljer
Main Authors: Stone, Orrin J., Biette, Kelly M., Murphy, Patrick J. M.
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2014
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4177899/
https://ncbi.nlm.nih.gov/pubmed/25254496
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0108611
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