Insertional mutagenesis and marker rescue in a protozoan parasite: cloning of the uracil phosphoribosyltransferase locus from Toxoplasma gondii.

Nonhomologous integration vectors have been used to demonstrate the feasibility of insertional mutagenesis in haploid tachyzoites of the protozoan parasite Toxoplasma gondii. Mutant clones resistant to 5-fluorouracil were identified at a frequency of approximately 10(-6) (approximately 2 x 10(-5) of...

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Εκδόθηκε σε:	Proc Natl Acad Sci U S A
Κύριοι συγγραφείς:	Donald, R G, Roos, D S
Μορφή:	Artigo
Γλώσσα:	Inglês
Έκδοση:	National Academy of Sciences 1995
Θέματα:	Research Article
Διαθέσιμο Online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC41774/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7777580/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.92.12.5749
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