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Room-temperature serial crystallography using a kinetically optimized microfluidic device for protein crystallization and on-chip X-ray diffraction

An emulsion-based serial crystallographic technology has been developed, in which nanolitre-sized droplets of protein solution are encapsulated in oil and stabilized by surfactant. Once the first crystal in a drop is nucleated, the small volume generates a negative feedback mechanism that lowers the...

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Autori principali: Heymann, Michael, Opthalage, Achini, Wierman, Jennifer L., Akella, Sathish, Szebenyi, Doletha M. E., Gruner, Sol M., Fraden, Seth
Natura: Artigo
Lingua:Inglês
Pubblicazione: International Union of Crystallography 2014
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4174877/
https://ncbi.nlm.nih.gov/pubmed/25295176
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2052252514016960
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