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Evaluation of amplification targets for the specific detection of Bordetella pertussis using real-time polymerase chain reaction

BACKGROUND: Bordetella pertussis infections continue to be a major public health challenge in Canada. Polymerase chain reaction (PCR) assays to detect B pertussis are typically based on the multicopy insertion sequence IS481, which offers high sensitivity but lacks species specificity. METHODS: A no...

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Main Authors: Hasan, Mohammad Rubayet, Tan, Rusung, Al-Rawahi, Ghada N, Thomas, Eva, Tilley, Peter
格式: Artigo
語言:Inglês
出版: Pulsus Group Inc 2014
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4173943/
https://ncbi.nlm.nih.gov/pubmed/25285127
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