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Determination of real-time polymerase chain reaction uncertainty of measurement using replicate analysis and a graphical user interface with Fieller’s theorem

BACKGROUND: Disease monitoring of viruses using real-time polymerase chain reaction (PCR) requires knowledge of the precision of the test to determine what constitutes a significant change. Calculation of quantitative PCR confidence limits requires bivariate statistical methods. OBJECTIVE: To develo...

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Detalhes bibliográficos
Main Authors: Stuart, James Ian, Delport, Johan, Lannigan, Robert, Zahariadis, George
Formato: Artigo
Idioma:Inglês
Publicado em: Pulsus Group Inc 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4173941/
https://ncbi.nlm.nih.gov/pubmed/25285125
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