Bacterial cytoplasm as an effective cell compartment for producing functional VHH-based affinity reagents and Camelidae IgG-like recombinant antibodies

BACKGROUND: The isolation of recombinant antibody fragments from displayed libraries represents a powerful alternative to the generation of IgGs using hybridoma technology. The selected antibody fragments can then be easily engineered into (multi)-tagged constructs of variable mass and complexity as...

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Detalhes bibliográficos
Main Authors: Djender, Selma, Schneider, Aurelie, Beugnet, Anne, Crepin, Ronan, Desrumeaux, Klervi Even, Romani, Chiara, Moutel, Sandrine, Perez, Franck, de Marco, Ario
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2014
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4172947/
https://ncbi.nlm.nih.gov/pubmed/25223348
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-014-0140-1
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