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Spectrophotometric Determination and Removal of Unchelated Europium Ions from Solutions Containing Eu-Diethylenetriaminepentaacetic Acid Chelate-Peptide Conjugates()

Europium chelates conjugated with peptide ligands are routinely used as probes for conducting in vitro binding experiments. The presence of unchelated Eu ions in these formulations gives high background luminescence and can lead to poor results in binding assays. In our experience, the reported meth...

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Detalhes bibliográficos
Main Authors: Dayan Elshan, N. G. R., Patek, Renata, Vagner, Josef, Mash, Eugene A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4172531/
https://ncbi.nlm.nih.gov/pubmed/25058927
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2014.07.009
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