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Library methods for structural biology of challenging proteins and their complexes

Genetic engineering of constructs to improve solubility or stability is a common approach, but it is often unclear how to obtain improvements. When the domain composition of a target is poorly understood, or if there are insufficient structure data to guide sited directed mutagenesis, long iterative...

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Detalles Bibliográficos
Autores principales: Hart, Darren J., Waldo, Geoffrey S.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2013
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4172339/
https://ncbi.nlm.nih.gov/pubmed/23602357
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.sbi.2013.03.004
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