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Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model

The ability to multiplex assays in studies of complex cellular mechanisms eliminates the need for repetitive experiments, provides internal controls, and decreases waste in costs and reagents. Here we describe optimization of a multiplex assay to assess apoptosis following a palmitic acid (PA) chall...

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Detalhes bibliográficos
Main Authors: Butterick, Tammy A., Duffy, Cayla M., Lee, Rachel E., Billington, Charles J., Kotz, Catherine M., Nixon, Joshua P.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2014
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4172056/
https://ncbi.nlm.nih.gov/pubmed/24797379
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51305
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