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Small scale affinity purification and high sensitivity reversed phase nanoLC-MS N-glycan characterization of mAbs and fusion proteins

N-glycosylation is a complex post-translational modification with potential effects on the efficacy and safety of therapeutic proteins and known influence on the effector function of biopharmaceutical monoclonal antibodies (mAbs). Comprehensive characterization of N-glycosylation is therefore import...

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Autors principals: Higel, Fabian, Seidl, Andreas, Demelbauer, Uwe, Sörgel, Fritz, Frieß, Wolfgang
Format: Artigo
Idioma:Inglês
Publicat: Landes Bioscience 2014
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4171024/
https://ncbi.nlm.nih.gov/pubmed/24848368
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/mabs.29263
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