Semi-automated protocol for purification of Mycobacterium leprae from tissues using the gentleMACS™ Octo Dissociator

Mycobacterium leprae, etiologic agent of leprosy, is propagated in athymic nude mouse footpads (FPs). The current purification protocol is tedious and physically demanding. A simpler, semi-automated protocol was developed using gentleMACS™ Octo Dissociator. The gentleMACS protocol provided a very ef...

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Autori principali: Williams, Diana L., Adams, Linda B., Lahiri, Ramanuj
Natura: Artigo
Lingua:Inglês
Pubblicazione: Elsevier Biomedical 2014
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4169666/
https://ncbi.nlm.nih.gov/pubmed/25019518
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mimet.2014.06.019
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