Genome-Scale Metabolic Network Validation of Shewanella oneidensis Using Transposon Insertion Frequency Analysis

Transposon mutagenesis, in combination with parallel sequencing, is becoming a powerful tool for en-masse mutant analysis. A probability generating function was used to explain observed miniHimar transposon insertion patterns, and gene essentiality calls were made by transposon insertion frequency a...

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Bibliografiske detaljer
Main Authors: Yang, Hong, Krumholz, Elias W., Brutinel, Evan D., Palani, Nagendra P., Sadowsky, Michael J., Odlyzko, Andrew M., Gralnick, Jeffrey A., Libourel, Igor G. L.
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2014
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4168976/
https://ncbi.nlm.nih.gov/pubmed/25233219
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pcbi.1003848
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