Phylogenomically Guided Identification of Industrially Relevant GH1 β-Glucosidases through DNA Synthesis and Nanostructure-Initiator Mass Spectrometry

[Image: see text] Harnessing the biotechnological potential of the large number of proteins available in sequence databases requires scalable methods for functional characterization. Here we propose a workflow to address this challenge by combining phylogenomic guided DNA synthesis with high-through...

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Main Authors: Heins, Richard A., Cheng, Xiaoliang, Nath, Sangeeta, Deng, Kai, Bowen, Benjamin P., Chivian, Dylan C., Datta, Supratim, Friedland, Gregory D., D’Haeseleer, Patrik, Wu, Dongying, Tran-Gyamfi, Mary, Scullin, Chessa S., Singh, Seema, Shi, Weibing, Hamilton, Matthew G., Bendall, Matthew L., Sczyrba, Alexander, Thompson, John, Feldman, Taya, Guenther, Joel M., Gladden, John M., Cheng, Jan-Fang, Adams, Paul D., Rubin, Edward M., Simmons, Blake A., Sale, Kenneth L., Northen, Trent R., Deutsch, Samuel
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2014
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4168791/
https://ncbi.nlm.nih.gov/pubmed/24984213
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/cb500244v
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