An Efficient Genotyping Method for Genome-modified Animals and Human Cells Generated with CRISPR/Cas9 System

The rapid generation of various species and strains of laboratory animals using CRISPR/Cas9 technology has dramatically accelerated the interrogation of gene function in vivo. So far, the dominant approach for genotyping of genome-modified animals has been the T7E1 endonuclease cleavage assay. Here,...

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Hauptverfasser: Zhu, Xiaoxiao, Xu, Yajie, Yu, Shanshan, Lu, Lu, Ding, Mingqin, Cheng, Jing, Song, Guoxu, Gao, Xing, Yao, Liangming, Fan, Dongdong, Meng, Shu, Zhang, Xuewen, Hu, Shengdi, Tian, Yong
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group 2014
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4168274/
https://ncbi.nlm.nih.gov/pubmed/25236476
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep06420
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