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Optimization of Extraction of Circulating RNAs from Plasma – Enabling Small RNA Sequencing

There are several protocols and kits for the extraction of circulating RNAs from plasma with a following quantification of specific genes via RT-qPCR. Due to the marginal amount of cell-free RNA in plasma samples, the total RNA yield is insufficient to perform Next-Generation Sequencing (NGS), the s...

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Detalhes bibliográficos
Main Authors: Spornraft, Melanie, Kirchner, Benedikt, Haase, Bettina, Benes, Vladimir, Pfaffl, Michael W., Riedmaier, Irmgard
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4167851/
https://ncbi.nlm.nih.gov/pubmed/25229651
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0107259
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