Limitations of Using Propidium Monoazide with qPCR to Discriminate between Live and Dead Legionella in Biofilm Samples

Accurately quantifying Legionella for regulatory purposes to protect public health is essential. Real-time PCR (qPCR) has been proposed as a better method for detecting and enumerating Legionella in samples than conventional culture method. However, since qPCR amplifies any target DNA in the sample,...

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Bibliographic Details
Main Authors: Taylor, Michael J, Bentham, Richard H, Ross, Kirstin E
Format: Artigo
Language:Inglês
Published: Libertas Academica 2014
Subjects:
Original Research
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4167484/
https://ncbi.nlm.nih.gov/pubmed/25288885
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4137/MBI.S17723
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