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Selection of chromosomal DNA libraries using a multiplex CRISPR system
The directed evolution of biomolecules to improve or change their activity is central to many engineering and synthetic biology efforts. However, selecting improved variants from gene libraries in living cells requires plasmid expression systems that suffer from variable copy number effects, or the...
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| Autori principali: | , , , , , , , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
eLife Sciences Publications, Ltd
2014
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4161972/ https://ncbi.nlm.nih.gov/pubmed/25139909 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.03703 |
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