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High-Throughput Bead-Based Identification of Structure-Switching Aptamer Beacons
We describe a new platform to identify structure-switching DNA beacon aptamers, which detect small molecules in a specific manner. By clonally amplifying a DNA library designed to fluoresce in response to binding events onto microbeads, aptamer beacons can be selected by stringent fluorescence-assis...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
WILEY-VCH Verlag
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4161366/ https://ncbi.nlm.nih.gov/pubmed/25056925 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201402037 |
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