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Application of Locked Nucleic Acid (LNA) Oligonucleotide–PCR Clamping Technique to Selectively PCR Amplify the SSU rRNA Genes of Bacteria in Investigating the Plant-Associated Community Structures
The simultaneous extraction of plant organelle (mitochondria and plastid) genes during the DNA extraction step is a major limitation in investigating the community structures of bacteria associated with plants because organelle SSU rRNA genes are easily amplified by PCR using primer sets that are sp...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Japanese Society of Microbial Ecology (JSME)/The Japanese Society of Soil Microbiology (JSSM)
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4159040/ https://ncbi.nlm.nih.gov/pubmed/25030190 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1264/jsme2.ME14061 |
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