Recombinant expression, purification, crystallization and preliminary X-ray diffraction analysis of the C-terminal DUF490(963–1138) domain of TamB from Escherichia coli

TamB is a recently described inner membrane protein that, together with its partner protein TamA, is required for the efficient secretion of a subset of autotransporter proteins in Gram-negative bacteria. In this study, the C-terminal DUF490(963–1138) domain of TamB was overexpressed in Escherichia...

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Detalhes bibliográficos
Main Authors: Josts, Inokentijs, Grinter, Rhys, Kelly, Sharon M., Mosbahi, Khedidja, Roszak, Aleksander, Cogdell, Richard, Smith, Brian O., Byron, Olwyn, Walker, Daniel
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2014
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4157435/
https://ncbi.nlm.nih.gov/pubmed/25195908
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X14017403
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