Crystallization, preliminary X-ray crystallographic and cryo-electron microscopy analysis of a bifunctional enzyme fucokinase/l-fucose-1-P-guanylyltransferase from Bacteroides fragilis

Fucokinase/l-fucose-1-P-guanylyltransferase (FKP) is a bifunctional enzyme which converts l-fucose to Fuc-1-P and thence to GDP-l-fucose through a salvage pathway. The molecular weights of full-length FKP (F-FKP) and C-terminally truncated FKP (C-FKP, residues 300–949) are 105.7 and 71.7 kDa, respec...

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Autors principals: Cheng, Chongyun, Gu, Jianhua, Su, Jing, Ding, Wei, Yin, Jie, Liang, Wenguang, Yu, Xiaoxia, Ma, Jun, Wang, Peng George, Xiao, Zhicheng, Liu, Zhi-Jie
Format: Artigo
Idioma:Inglês
Publicat: International Union of Crystallography 2014
Matèries:
Crystallization Communications
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4157419/
https://ncbi.nlm.nih.gov/pubmed/25195892
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X14012722
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