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Fluorescent TEM-1 β-lactamase with wild-type activity as a rapid drug sensor for in vitro drug screening
We report the development of a novel fluorescent drug sensor from the bacterial drug target TEM-1 β-lactamase through the combined strategy of Val(216)→Cys(216) mutation and fluorophore labelling for in vitro drug screening. The Val(216) residue in TEM-1 is replaced with a cysteine residue, and the...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Portland Press Ltd.
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4155835/ https://ncbi.nlm.nih.gov/pubmed/25074398 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BSR20140057 |
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