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Optimizing the fluorescent yield in two-photon laser scanning microscopy with dispersion compensation

A challenge for nonlinear imaging in living tissue is to maximize the total fluorescent yield from each fluorophore. We investigated the emission rates of three fluorophores – rhodamine B, a red fluorescent protein, and CdSe quantum dots – while manipulating the phase of the laser excitation pulse a...

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Bibliografiske detaljer
Main Authors: Field, Jeffrey J., Carriles, Ramón, Sheetz, Kraig E., Chandler, Eric V., Hoover, Erich E., Tillo, Shane E., Hughes, Thom E., Sylvester, Anne W., Kleinfeld, David, Squier, Jeff A.
Format: Artigo
Sprog:Inglês
Udgivet: 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4151303/
https://ncbi.nlm.nih.gov/pubmed/20588500
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