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Generation of a transgenic ORFeome library in Drosophila
Overexpression screens can be used to explore gene function in Drosophila melanogaster, but to demonstrate their full potential comprehensive and systematic collections of fly strains are required. Here we provide a protocol for high-throughput cloning of Drosophila open reading frames (ORFs) regula...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4150248/ https://ncbi.nlm.nih.gov/pubmed/24922270 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.105 |
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