Characterization of an Alginate Lyase, FlAlyA, from Flavobacterium sp. Strain UMI-01 and Its Expression in Escherichia coli

A major alginate lyase, FlAlyA, was purified from the periplasmic fraction of an alginate-assimilating bacterium, Flavobacterium sp. strain UMI-01. FlAlyA showed a single band of ~30 kDa on SDS-PAGE and exhibited the optimal temperature and pH at 55 °C and pH 7.7, respectively. Analyses for substrat...

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Bibliographic Details
Main Authors: Inoue, Akira, Takadono, Kohei, Nishiyama, Ryuji, Tajima, Kenji, Kobayashi, Takanori, Ojima, Takao
Format: Artigo
Language:Inglês
Published: MDPI 2014
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4145338/
https://ncbi.nlm.nih.gov/pubmed/25153766
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/md12084693
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